Detection of transgenes in genetically modified soybean and maize using polymerase chain reaction

Polymerase chain reaction (PCR) has been successfully employed to detect transgenes in genetically modified (GM) soybean and maize. Three pairs of primers for CaMV 35S, NOS and EPSPS genes were used in the detection. The results reveal that PCR could differentiate the GM plants from non-GM plants. Evidently, PCR is a highly reproducible and sensitive technique that can be successfully employed in detecting transgenes for screening GM soybeans and GM maize from their conventional plants.